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Zeiss AxioCam MR/HR Adapter
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Summary: | Interfaces Zeiss AxioCam medium resolution and high resolution color and monochrome cameras |
Author: | Henry Pinkard |
License: | Source code cannot be made available |
Platforms: | Windows |
Devices: | AxioCam MRm/MRc/HRm/HRc, MRm/MRc/HRm/HRc Rev. 2, and MRm/MRc/HRm/HRc Rev. 3 (tested with MRm Rev. 2, MRm Rev. 3, MRc Rev. 3, HRm Rev. 2, HRm Rev. 3); MRc5 |
Because of built in delays in the AxioCam's image acquisition, there is a delay of approximately 200ms between when Micro-Manager sends the command to open the shutter and when the AxioCam's exposure begins. If over exposure of the sample is a concern, it is highly recommended that hardware triggering is used with these cameras. Refer to the below descriptions of hardware triggering to do so (All images and text below ©2010 Carl Zeiss MicroImaging GmbH. All rights reserved.).
Trigger-Out
Via a supplementary cable the AxioCam has the capability of sending out aTTL trigger pulse to an external device, for example a mechanical shutter. Thistrigger pulse can be used to synchronizethe exposure of the camera and the task of the external device, as you canspecify the delay between sending outthe trigger signal and actually starting the acquisition.
To meet the requirements of the device you are using, you caninvert the polarity that you want thesignal to have.
Trigger-In

The exact time of exposure can be triggered from outside the camera. When the trigger-in is enabled and an acquisition function is called, the camera will delay the acquisition and wait for a trigger-in signal to be sent from an external device until it invokes the exposure.The following graphic demonstrates the two camera models' reaction to the trigger signal when performing a triggered acquisition. An acquisition request is sent out at each T, the acquisition takes place at an A:
NOTE: The trigger OUT of the AxioCam HR / MR expects a TTL signal.
Carl Zeiss Imaging 1394 Driver Downloads
Trigger Timing
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Due to different hardware and trigger mechanisms, the precise calculation ofthe camera's timing when involvong the trigger IN / OUT is a bit difficult anddepends on the camera model's internal design.
The following charts demonstrate, how an example acquisition using bothtrigger IN and trigger OUT could look like and explain how to preciselycalculate the timing.
AxioCam HR / MR |
The AxioCam HR / MR accepts only positive values for the trigger OUT delay, ranging from 0 to 255 row cyles, where 1 cycle lasts 154 µs (AxioCam HR) respectively 99 µs (AxioCam MR).
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AxioCam MRc5 / MR Rev.3 / HS |
The AxioCam MRc5 / MR Rev.3 / HS allows both positive and negative values for the trigger OUT delay, ranging from +4095 row cycles to -4095 row cyles, where 1 cycle lasts 244 µs. |
- negative delay |
If the specified trigger OUT delay is longer than the interval for the fixed idle rows and clearing the sensor, the trigger OUT is sent directly after the initialization has been completed. The camera performs the cleanoutand then waits an appropriate amount of time until the exposition is invoked.
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If the specified trigger OUT delay is shorter than the interval for the fixed idle rows and clearing the sensor, the camera starts the sensor cleanout right after the initialization was finished and delays sending the triggerOUT signal to achieve the desired trigger OUT delay.
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- positive delay |
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--Henry Pinkard 10:58, 2nd January 2013 (PDT)
IEEE 1394 driver on Windows 7
Carl Zeiss Imaging 1394 Driver Download
Windows 7 comes with two versions of the IEEE 1394 bus driver (default and 'legacy'). I had trouble getting an AxioCam MRc5 recognized with the default one. This might be dependent on the motherboard and IEEE 1394 adapter. To switch between the default and 'legacy' drivers, follow the instructions mentioned here: http://social.technet.microsoft.com/Forums/en-US/itprovistahardware/thread/c2e39b2c-0558-43cb-865e-9f9db3e4f5ed/
Using the Legacy driver may also help with cases where the message '(0)McamAcquisition error 22' pops up and Micro-Manager becomes unresponsive.

--Mark Tsuchida
Carl Zeiss Imaging 1394 Driver Download Windows 10
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